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Table 1 Structural parameters and data fitting of PcaLOOL proteins from SEC-SAXS

From: SANS investigation of fungal loosenins reveals substrate-dependent impacts of protein action on the inter-microfibril arrangement of cellulosic substrates

 

PcaLOOL12

PcaLOOL7

PcaLOOL9

Guinier analysis

 I (0) (a.u)

82.7 ± 0.4

109.7 ± 0.3

93.5 ± 0.1

 Rg (Å)

15.7 ± 0.2

17.1 ± 0.1

15.7 ± 0.1

 Q Rgmax

0.20- 1.28

0.23–1.29

0.30–1.30

P(r) analysisa

 bI (0) (a.u)

83.8 ± 0.5

109.8 ± 0.3

94.6 ± 0.2

 Rg (Å)

16.5 ± 0.3

17.9 ± 0.2

16.4 ± 0.1

 Dmax (Å)

60

72

91

 cQ range (Å−1)

0.012–0.3

0.14–0.5

0.013–0.4

 χ2

1.2

 < 1.0

1.0

Molecular weight (MW) analysis

 MW from Porod volume (kDa)

12.6

14.9

11.9

 dCalculated MW (kDa)

11.4

13.4

12.0

  1. aP (r) profiles are shown in Figure S10; bI (0) from SEC-SAXS is not on absolute scale; cQ range consists of Qmin to Qmax; dMolecular weights were calculated from their primary amino acid sequences