Fig. 5

Evolutionary relationships of Cortinarius OMTs. OMTs yielding dermolutein (8-O-methylendocrocin) are in green; those able to produce physcion (6-O-methylemodin) are in pink; those affording questin (8-O-methylemodin) are in blue; while selected putative OMTs orthologous to these functionally characterized enzymes are shown in black. Note that OMTs able to produce physcion in synthetic biology systems may yield different products when part of their native biosynthetic pathways in their native producer organisms. The evolutionary history of the tree was inferred using the neighbor-joining method with a bootstrap test of 1,000, with the caffeic acid OMT (Ref_COMT) from Zea mays as the outgroup. See SI Table S13 for the GenBank accessions of the sequences