Transcriptome analysis and reverse engineering verification of SNZ3Val125Ile and Pho3Asn134Asp revealed the mechanism of adaptive laboratory evolution to increase the yield of tyrosol in Saccharomyces cerevisiae strain S26-AE2

Table 1 Proteins with SNP mutations contained in strain S26-AE2

Gene description	Chrom	Genome position	Ref	Alt	Anno	Gene description	Type	Mutations
MAL32	CM001523.1	800665	T	C	Exonic	Synonymous SNV	SNP	Phe472Phe
MAL32	CM001523.1	800863	G	A	Exonic	Synonymous SNV	SNP	Leu538Leu
SNZ3	CM001527.1	8368	C	T	Exonic	Synonymous SNV	SNP	Gly93Gly
SNZ3	CM001527.1	8416	C	T	Exonic	Synonymous SNV	SNP	Ile109Ile
SNZ3	CM001527.1	8443	C	T	Exonic	Synonymous SNV	SNP	Asn118Asn
SNZ3	CM001527.1	8455	A	G	Exonic	Synonymous SNV	SNP	Leu122Leu
SNZ3	CM001527.1	8462	G	A	Exonic	Nonsynonymous SNV	SNP	Val125Ile
DOT6	CM001526.1	332037	T	G	Exonic	Nonsynonymous SNV	SNP	Lys355Asn
DOT6	CM001526.1	332040	C	A	Exonic	Nonsynonymous SNV	SNP	Arg354Ser
HXT3	CM001525.1	1161695	A	C	Exonic	Synonymous SNV	SNP	Val538Val
PHO3	CM001523.1	422454	T	C	Exonic	Nonsynonymous SNV	SNP	Asn134Asp
PHO3	CM001523.1	422458	T	C	Exonic	Synonymous SNV	SNP	Ser132Ser
PHO3	CM001523.1	421594	A	G	Exonic	Synonymous SNV	SNP	Asp420Asp

1. ''Chrom'' means chromosome. 2. ''Ref'' means Reference sequence. 3. ''Alt'' means Alternative sequence. 4. ''Anno'' means Annotation

ISSN: 2731-3654