Fig. 2

Characterization of DAL promoters induced by allantoin. A Schematic diagram of plasmid composition for characterization of the strength of DAL promoters. The engineered yeast cells were cultivated in normal YNBD medium (repression state) or YABD medium (activation state). B Comparison of EGFP expressions under the control of DAL2/DAL3 promoter in normal YNBD medium (repression state) or YABD medium (activation state). C The cell growth profile of BY4741-derived strains in normal YNBD medium (repression state) or YABD medium (activation state). All experiments were performed in triplicate, and data represent the mean values with standard deviations