Fig. 3
From: Design and characterization of allantoin-inducible expression systems in budding yeast
Characterization of allantoin–GAL expression system with signal amplification effect. A Schematic design of the allantoin–GAL system. By deleting the Gal80 gene (encoding the negative regulator of Gal4 transcriptional activator) and replacing the Gal4 native promoter with the DAL2 promoter in BY4741 strain, the input signal from allantoin inducer can be amplified by Gal4, which binds to the upstream activation sequences (UASG) of GAL genes and would eventually result in a stronger signal output of GOI under the GAL promoter. Once the Gal4 transcriptional activator is expressed, it can persistently drive the GOI expression under the GAL promoters. GOI, genes-of-interest. B The EGFP expression of engineered yeast cells in normal YNBD medium (repression state) or YABD medium (activation state). Strain BY4741 transformed with plasmid pRS425DAL2-EGFP and strain JS-BE5-Allan transformed with plasmid pRS415Gal1-EGFP and pRS425Gal1-EGFP was used to collect the data. All experiments were performed in triplicate, and data represent the mean values with standard deviations