Fig. 2
Initial establishment and expansion of the CmeR-PcmeO biosensor system. a Schematic representation of the re-established CmeR-PcmeO biosensor system. The CmeR protein binds to PcmeO to inhibit promoter expression, while salicylic acid (SA) interacts with the CmeR protein to alleviate this repression. b A systematic combinatorial analysis and optimization of cmeO for CmeR expression were conducted. c Dynamic performance of the wild-type CmeR-PcmeO biosensor system in E. coli W3110 was assessed. d, e Optimization of the CmeR-PcmeO biosensor system through cmeO binding sites was performed, and the data were obtained after 18Â h of cultivation in 24-well plates