Fig. 3
Further optimization of the CmeR-PcmeO biosensor system. a, b A systematic combinatorial analysis and optimization of the promoter and ribosome binding site (RBS) for CmeR expression were conducted using constitutive promoters with established relative strengths (http://parts.igem.org/Promoters/Catalog/Ecoli/Constitutive). c Discovery Studio was utilized to simulate the CmeR-SA complex, followed by validation and analysis using molecular docking software AutoDock Vina and ChimeraX 1.8, revealing the interactions between CmeR residues and SA. d Fluorescence signals of various mutants of CmeR were analyzed