Table 1 Engineering strains used in this study

W3110

Lab stock

 W3110K-0.1

W3110

pTrc-P_trc-entC-pchB

This study

 W3110K-0.2

W3110K-0.1

pACYCD-P_trc-aroG^s180F-ppsA-tktA-aroL

This study

 W3110K-1

W3110K-0.2

ΔpykA/F

This study

 W3110K-1.1

W3110

ΔpykA::P_trc-lao-entC-pchB

This study

 W3110K-1.2

W3110K-1.1

yjiV::P_trc-lao-ppsA

This study

 W3110K-1.3

W3110K-1.2

ygaY::P_trc-lao-tktA

This study

 W3110K-1.4

W3110K-1.3

yeeP::P_trc-lao-aroL

This study

 W3110K-2

W3110K-1.4

yneU::P_trc-lao-aroG^S180F

This study

 W3110K-3

W3110K-2

ALE

This study

 W3110K-4

W3110K-3

FACS

This study

 W3110K-4.1

W3110K-4

ΔP_trc-lao-entC-pchB:: P_J23114-lao-cmeR^F99A

This study

 W3110K-4.11

W3110K-4.1

pTarget-P_trc-entC-pchB-J23119-trpE::sg1-P_O1O3-Dcas9; pTarget-P_trc-entC-pchB-J23119-trpE::sg2-P_O1O3-Dcas9; pTarget-P_trc-entC-pchB-J23119-trpE::sg3-P_O1O3-Dcas9; pTarget-P_trc-entC-pchB-J23119-trpE::sg4-P_O1O3-Dcas9

This study

 W3110K-4.2

W3110K-4.11

pTarget-P_trc-entC-pchB-J23119-trpE::sg3-P_O1O3-Dcas9

This study

 W3110K-4.21

B3110K-4.2

pTarget-P_trc-entC-pchB-J23119-trpE pheA::sg3-sg1-P_O1O3-Dcas9; pTarget-P_trc-entC-pchB-J23119-trpE pheA::sg3-sg2-P_O1O3-Dcas9; pTarget-P_trc-entC-pchB-J23119-trpE pheA::sg3-sg3-P_O1O3-Dcas9; pTarget-P_trc-entC-pchB-J23119-trpE pheA::sg3-sg4-P_O1O3-Dcas9

This study

 W3110K-4.3

W3110K-4.21

pTarget-P_trc-entC-pchB-J23119-trpE pheA::sg3-sg1-P_O1O3-Dcas9

This study

 W3110K-4.31

W3110K-4.3

pTarget-P_trc-entC-pchB-J23119-trpE pheA tyrA::sg3-sg1-sg1-P_O1O3-Dcas9; pTarget-P_trc-entC-pchB-J23119-trpE pheA tyrA::sg3-sg1-sg2-P_O1O3-Dcas9;pTarget-P_trc-entC-pchB-J23119-trpE pheA tyrA::sg3-sg1-sg3-P_O1O3-Dcas9; pTarget-P_trc-entC-pchB-J23119-trpE pheA tyrA::sg3-sg1-sg4-P_O1O3-Dcas9

This study

 W3110K-5

W3110K-4.31

pTarget-P_trc-entC-pchB-J23119-trpE pheA tyrA::sg3-sg1-sg3-P_O1O3-Dcas9

This study

 W3110K-W2

W3110K-2

pTarget- P_trc-entC-pchB-J23119-trpE pheA tyrA::sg3-sg1-sg3-P_O1O3-Dcas9

This study

 W3110AE-1

W3110K-4

ΔybfC^ALE

This study

 W3110B-1

W3110 AE-1

pACYCD-P_trc-ybfC^ALE

This study

 W3110AE-2

W3110K-4

Δadhp^ALE

This study

 W3110B-2

W3110AE-2

pACYCD-P_trc-adhp^ALE

This study

 W3110AE-3

W3110K-4

ΔmlC^ALE

This study

 W3110B-3

W3110AE-3

pACYCD-P_trc-mlC^ALE

This study

 W3110AE-4

W3110K-4

ΔompC^ALE

This study

 W3110B-4

W3110AE-4

pACYCD-P_trc-ompC^ALE

This study

 W3110AE-5

W3110K-4

ΔhcaD^ALE

This study

 W3110B-5

W3110AE-5

pACYCD-P_trc-hcaD^ALE

This study

 W3110AE-6

W3110K-4

ΔaaeB^ALE

This study

 W3110B-6

W3110AE-6

pACYCD-P_trc-aaeB^ALE

This study

 W3110AE-7

W3110K-4

ΔpmrB^ALE

This study

 W3110B-7

W3110AE-7

pACYCD-P_trc-pmrB^ALE

This study

 W3110AE-8

W3110K-4

ΔaspA^ALE; ΔfxsA^ALE; yjeH^ALE

This study

 W3110B-8

W3110AE-8

pACYCD-P_trc-aspA^ALE-ΔfxsA^ALE- yjeH^ALE

This study

 W3110AE-9

W3110K-4

ΔtreB^ALE

This study

 W3110B-9

W3110AE-9

pACYCD-P_trc-treB^ALE

This study

 W3110AE-10

W3110K-4

ΔompT^ALE

This study

 W3110B-10

W3110AE-10

pACYCD-P_trc-ompT^ALE

This study

 W3110AE-11

W3110K-4

ΔymdA^ALE; ΔymdB^ALE; ΔclsC^ALE; ΔcsgB^ALE; ΔcsgA^ALE

This study

 W3110B-11

W3110AE-11

pACYCD-P_trc-ymdA^ALE-ymdB^ALE-clsC^ALE-csgB^ALE-csgA^ALE

This study

 W3110AE-12

W3110K-4

ΔducA^ALE

This study

 W3110B-12

W3110AE-12

pACYCD-P_trc-ducA^ALE

This study

 W3110C-1

W3110K-2

ΔP_trc-lao-entC-pchB:: P_J23114-lao-cmeR^F99A

This study

 W3110C-21

W3110C-1

pTarget-P_trc-entC-pchB-J23119-trpE pheA tyrA::sg3-sg1-sg3-P_O1O3-Dcas9

This study

 W3110VC-1

W3110C-1

pACYCD-P_trc-ymdA^ALE-ymdB^ALE-clsC^ALE-csgB^ALE-csgA^ALE

This study

 W3110C-1.1

W3110C-1

pACYCD-P_trc-ymdA^ALE-ymdB^ALE-clsC^ALE-csgB^ALE-csgA^ALE-ymdA

This study

 W3110C-2

W3110C-1.1

pTarget-P_trc-entC-pchB-J23119-trpE pheA tyrA::sg3-sg1-sg3-P_O1O3-Dcas9

This study

 W3110BC-3

W3110K-2

pACYCD-P_trc-aspA^ALE-ΔfxsA^ALE- yjeH^ALE

This study

 W3110C-3

W3110K-2

pACYCD-P_trc-aspA^ALE-ΔfxsA^ALE- yjeH^ALE -ompT

This study