Fig. 2

Schematic representation of pPtGE30 expression vector and insertion of CsTKS and CsOAC cassettes. The pTKS plasmid includes a construction that contains the 1971-bp cassette with CsTKS under control of native constitutive 40SRPS8 promoter, 6xHis marker and an FcpA terminator. The pOAC plasmid harbors a construction with the 1265 bp cassette CsOAC under control of FcpD promoter, marked with a Myc tag, and ended with an FcpD terminator. The Cen-His-Ars region is necessary for episomal replication while the OriT permits the transfer of the plasmid in P. tricornutum during interkingdom conjugation. Finally, the Sh ble and CmR coding sequence allows for zeocin resistance when expressed in P. tricornutum and chloramphenicol resistance in E. coli, respectively