Impact of heterologous expression of Cannabis sativa tetraketide synthase on Phaeodactylum tricornutum metabolic profile

Table 1 Impact of heterologous CsTKS on P. tricornutum’s metabolism

Analyte	Family	Biosynthesis/metabolic route
Norvaline Betaine	Amino Acid dipeptide	Pyruvate Isoleucine pathway and choline oxidation reactions [44, 46,47,48]
Thiamine*	Vitamin/Cofactor	Vitamin B6, glycine, pyruvate and purine metabolism [44, 49]
Quinolizine	Alkaloid/Quinolizine	Unsolved, possible pyridine derived precursor, Malonyl-CoA and a Polyketide synthase [50]
Methyl 2-[(4-methyl-2-oxo-2H-chromen-7-yl)oxy]propanoate	Coumarin Derivative	Shikimate Pathway [44, 51]
Thiamine Pyrophosphate	Vitamin/Cofactor	Pyruvate and purine metabolism [44, 49]
Alanylphenylalanine	Amino Acid dipeptide	Shikimate Pathway [44, 52]
2-Hydroxyacetophenone	Organic aromatic alkyl-phenylketones/Benzoic Acid	Putative Shikimate and polyketide Pathway [53]
Guanine	Nucleic Acid	Nucleotide metabolism [44, 54, 55]
Thermopsine	Alkaloid/Quinolizidine	Lysine decarboxylation [50, 56]

The presence of CsTKS alters the activity of key metabolic pathways, including the shikimate pathway, nucleotide metabolism, and amino acid metabolism. Metabolites highlighted in pink-orange are all upregulated, while those in blue are downregulated. Analytes are listed in descending order of fold change. The “Biosynthesis/metabolic route” column emphasizes shared pathways or biosynthetic routes among the identified analytes

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