Fig. 1

Biochemical and functional characterization of AeGE15 and TlGE15. SDS–PAGE of AeGE15 and TlGE15 (A) and TLC analysis of the GEs reaction mixtures using trans−3-phenyl-2-propen-1-yl D-glucopyranosyluronate as substrate, visualized under UV (B) and with N-(1-Naphthyl)-ethylenediamine dihydrochloride (C). Lanes in panel (A); Purified AeGE15 (1) and TlGE15 (3), and AeGE15 (2) and TlGE15 (4) after treatment with Endo H, LMW standard protein markers (S). Lanes in panels (B, C); Cinnamyl alcohol (CA), glucuronic acid (GlcA), control reaction containing the substrate without any enzyme (Control), and reactions using the purified GEs AeGE15 (Rxn_1) and TlGE15 (Rxn_2)