Fig. 2

Visualization of the structural models of AeGE15 (cyan) and TlGE15 (yellow), comparing to the crystal structure of StGE15–S213A mutant (pink) in complex with methyl 4-O-methyl-β-D-glucopyranuronate (representation in grey sticks; PDB: 4G4J). A Structural models of the catalytic domain of AeGE15 and TlGE15 demonstrate high similarity to StGE15–S213A. The models of AeGE15 and TlGE15 were generated using AlphaFold [21]. Catalytic amino acids are marked and represented in sticks. The active sites of AeGE15 (B) and TlGE15 (C) reserve the amino acids that interact with the ligand (annotated and represented in sticks) in the StGE15–S213A structure