Fig. 4
From: Two routes for tyrosol production by metabolic engineering of Corynebacterium glutamicum
Tyrosol production with native 4-OH-phenylpyruvate synthesis by strain AROM3 (ARO10Sc). A Tyrosol synthesis pathway with 4-OH-phenylpyruvate as intermediate, synthesized via prephenate dehydrogenase from E. coli (heterologously expressed by tyrAEcfbr) or via native aminotransferases (encoded by undefined genes at and putative dapC) from l-tyrosine. B Conversion of l-tyrosine to tyrosol by C. glutamicum AROM3 (ARO10Sc) in a whole-cell enzyme assay with permeabilized cells upon supplementation of 3 mM l-tyrosine (l-Tyr) and surplus addition of amino acceptors pyruvate (Pyr) or 2-oxoglutarate (2-OG), as indicated. C Influence of plasmid-based overexpression of dapC on growth (CDW) and production of l-tyrosine and tyrosol by strain C. glutamicum AROM3 (ARO10Sc). Glucose was depleted after 72 h by the empty vector strain AROM3 (ARO10Sc)(EV3) and after 120 h by AROM3 (ARO10Sc)(dapC). Values and error bars represent means and standard deviations of triplicate cultivations