Fig. 6
From: Two routes for tyrosol production by metabolic engineering of Corynebacterium glutamicum
Tyrosol production via plasmid-based expression of tyoKr in strain AROM3 (tdcLb) and combination with ARO10Sc. A Tyrosol synthesis pathway with tyramine as intermediate, including the overexpression of the heterologous genes tdcLb and tyoKr (green), encoding l-tyrosine decarboxylase from L. brevis and tyramine oxidase from K. rhizophila, respectively, overexpression of ARO10Sc and the involvement of the furfural dehydrogenase encoded by fudC (among other alcohol dehydrogenases). B, C Comparison of growth (CDW) and production of l-tyrosine, tyramine, and tyrosol for C. glutamicum AROM3 (tdcLb)(tyoKr) (B) (glucose was exhausted after 48 h) and for the strain with additional overexpression of ARO10Sc (C) (glucose was depleted after 72 h). Values and error bars represent means and standard deviations of triplicate shake flask cultivations