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Table 4 Result of the thermostability analysis for tools that have as input the AMBGL18 sequence and, as output, only the information whether the mutation is stabilizing (S) or destabilizing (D)

From: Development of a β-glucosidase improved for glucose retroinhibition for cellulosic ethanol production: an integrated bioinformatics and genetic engineering approach

 

Alanine

Cysteine

Aspartic acid

Glutamic acid

Phenylalanine

Mupro (SEQ)

D

D

D

D

D

iStable (SEQ)

D

D

D

D

D

MuStab (SEQ)

D

D

D

D

D

 

Glycine

Histidine

Isoleucine

Lysine

Leucine

Mupro (SEQ)

S

D

D

D

S

iStable (SEQ)

D

D

D

D

S

MuStab (SEQ)

D

D

D

D

D

 

Methionine

Asparagine

Proline

Glutamine

Arginine

Mupro (SEQ)

S

D

D

D

D

iStable (SEQ)

S

D

D

D

D

MuStab (SEQ)

D

D

D

D

D

 

Serine

Threonine

Valine

Tyrosine

 

Mupro (SEQ)

D

D

S

D

 

iStable (SEQ)

D

D

S

D

 

MuStab (SEQ)

D

D

D

D