Fig. 4

Effects of three 5’UTRs on reporter gene translation respectively and transactivation. a Measurement of GUS enzyme activity and GUS staining was performed on leaves with a diameter of one centimeter, and the observed color variations indicate differential effects of different UTRs on GUS translation. Different letters above the bars indicate significantly different values (p < 0.05) calculated by one-way analysis of variance (ANOVA) followed by Tukey’s multiple range. b The fluorescence intensity indicates differential effects of different UTRs on eGFP translation. Scale bars = 100 μm. c Subcellular localization of two BnABF4L-CDS. Scale bars = 25 μm d Assessment of the transcriptional activation activity was carried out on two BnABF4L-CDS proteins. The pGBKT7 vector and GAL4 gene were utilized as negative and positive controls, respectively. The measurement of β-galactosidase activities was conducted through X-α-gal staining