Fig. 3
Source data can be found at Additional File 1, Table S4 and Table S5
Characterization of the ΔrexA mutant in heterotrophic batch fermentation. A, B Growth and product formation of wild type (A) and ΔrexA (B) in batch fermentation with excess glucose (50 g L−1). Data represent three biological replicates (average ± standard deviation). The maximum specific growth rate µmax was calculated from time point 43 and 53 for the wild type and from time point 31 and 40 for ΔrexA. C Results of RNA-seq analysis are shown as heatmap of the ten most differentially expressed genes in ΔrexA compared to the wild type. Log2 fold change differences (> ± 1.5-fold) between wild type and ΔrexA (blue: downregulation, red: upregulation) as well as TPM values from triplicates are shown. Locus tags are from the G-1 reference genome (OZ020628) [9]. Genes without formal gene name are displayed per the first word of the product name (LytTR = LytTR family DNA-binding domain-containing protein, GHLK = GHKL domain-containing protein). A grey line separates the genes found in the rexA locus from those involved in sugar metabolism. D Top: Sequence logo generated with the consensus sequence for Thermoanaerobacterales from the RegPrecise database [54]. Bottom: Schematic representation of the genetic architecture of a subset of differentially expressed genes in T. kivui and T. kivui ΔrexA. Colors are taken from (C) for log2-fold expression. Yellow diamond: Rex recognition motif. Arrow: Promoter.