Table 2 PB design matrix for screening independent variables with actual and coded values influencing hydrogen production
Trial | Variables | Response | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | Hydrogen production (mLL−1) | ||
Initial pH | Incubation temperature (°C) | Inoculum volume (%) | WMP (%) | Sodium acetate (gL−1) | Ammonium acetate (gL−1) | Tryptone (gL−1) | Yeast extract (gL−1) | Experimental | Predicted | |
1 | 1 (8) | − 1 (28) | − 1 (5) | − 1 (10) | − 1 (1) | 1 (5) | − 1 (1) | 1 (3) | 827.00 | 1136.22 |
2 | 1 (8) | 1 (37) | − 1 (5) | − 1 (10) | − 1 (1) | − 1 (1) | 1 (7) | − 1 (0.5) | 1171.67 | 1734.44 |
3 | 1 (8) | 1 (37) | 1 (25) | − 1 (10) | − 1 (1) | − 1 (1) | − 1 (1) | 1 (3) | 1865.00 | 1610.78 |
4 | 1 (8) | 1 (37) | 1 (25) | 1 (40) | − 1 (1) | − 1 (1) | − 1 (1) | − 1 (0.5) | 2630.00 | 2863.39 |
5 | 1 (8) | 1 (37) | 1 (25) | 1 (40) | 1 (4) | − 1 (1) | − 1 (1) | − 1 (0.5) | 3280.33 | 3423.33 |
6 | − 1 (5) | 1 (37) | 1 (25) | 1 (40) | 1 (4) | 1 (5) | − 1 (1) | − 1 (0.5) | 265.67 | 353.06 |
7 | 1 (8) | − 1 (28) | 1 (25) | 1 (40) | 1 (4) | 1 (5) | 1 (7) | − 1 (0.5) | 3607.67 | 2874.17 |
8 | − 1 (5) | 1 (37) | − 1 (5) | 1 (40) | 1 (4) | 1 (5) | 1 (7) | 1 (3) | 160.33 | 297.72 |
9 | 1 (8) | − 1 (28) | 1 (25) | − 1 (10) | 1 (4) | 1 (5) | 1 (7) | 1 (3) | 995.33 | 1221.56 |
10 | 1 (8) | 1 (37) | − 1 (5) | 1 (40) | − 1 (1) | 1 (5) | 1 (7) | 1 (3) | 2652.67 | 2028.61 |
11 | − 1 (5) | 1 (37) | 1 (25) | − 1 (10) | 1 (4) | − 1 (1) | 1 (7) | 1 (3) | 255.00 | 184.67 |
12 | − 1 (5) | − 1 (28) | 1 (25) | 1 (40) | − 1 (1) | 1 (5) | − 1 (1) | 1 (3) | 245.00 | 179.11 |
13 | 1 (8) | − 1 (28) | − 1 (5) | 1 (40) | 1 (4) | − 1 (1) | 1 (7) | − 1 (0.5) | 3512.00 | 3333 |
14 | 1 (8) | 1 (37) | − 1 (5) | − 1 (10) | 1 (4) | 1 (5) | − 1 (1) | 1 (3) | 1701.33 | 1670.67 |
15 | − 1 (5) | 1 (37) | 1 (25) | − 1 (10) | − 1 (1) | 1 (5) | 1 (7) | − 1 (0.5) | 210.67 | 183.33 |
16 | − 1 (5) | − 1 (28) | 1 (25) | 1 (40) | − 1 (1) | − 1 (1) | 1 (7) | 1 (3) | 395.00 | 463.33 |
17 | 1 (8) | − 1 (28) | − 1 (5) | 1 (40) | 1 (4) | − 1 (1) | − 1 (1) | 1 (3) | 3680.00 | 3188.72 |
18 | − 1 (5) | 1 (37) | − 1 (5) | − 1 (10) | 1 (4) | 1 (5) | − 1 (1) | − 1 (0.5) | 220.00 | 110.67 |
19 | 1 (8) | − 1 (28) | 1 (25) | − 1 (10) | − 1 (1) | 1 (5) | 1 (7) | − 1 (0.5) | 863.00 | 1301.11 |
20 | − 1 (5) | 1 (37) | − 1 (5) | 1 (40) | − 1 (1) | − 1 (1) | 1 (7) | 1 (3) | 275.33 | 230.51 |
21 | − 1 (5) | − 1 (28) | 1 (25) | − 1 (10) | 1 (4) | − 1 (1) | − 1 (1) | 1 (3) | 352.00 | 205.39 |
22 | − 1 (5) | − 1 (28) | − 1 (5) | 1 (40) | − 1 (1) | 1 (5) | − 1 (1) | − 1 (0.5) | 215.67 | 398 |
23 | − 1 (5) | − 1 (28) | − 1 (5) | − 1 (10) | 1 (4) | − 1 (1) | 1 (7) | − 1 (0.5) | 171.00 | 229.06 |
24 | − 1 (5) | − 1 (28) | − 1 (5) | − 1 (10) | − 1 (1) | − 1 (1) | − 1 (1) | − 1 (0.5) | 130.33 | 84.67 |
25 | 0 (6.5) | 0 (32) | 0 (15) | 0 (25) | 0 (2.5) | 0 (3) | 0 (4) | 0 (1.5) | 2055.33 | 1825.46 |
26 | 0 (6.5) | 0 (32) | 0 (15) | 0 (25) | 0 (2.5) | 0 (3) | 0 (4) | 0 (1.5) | 1898.67 | 1825.46 |
27 | 0 (6.5) | 0 (32) | 0 (15) | 0 (25) | 0 (2.5) | 0 (3) | 0 (4) | 0 (1.5) | 1800.33 | 1825.46 |