Maximizing biohydrogen production from watermelon peels using Clostridium butyricum NE133: a statistical optimization approach with Plackett–Burman and Box–Behnken designs

Table 5 Analysis of variance (ANOVA) and results of regression analysis of BB design for hydrogen production

Source	DF	Adj SS	Adj MS	F-value	P-value	Significance
Model	14	7,672,586	548,042	86.85	0.000	HS*
Linear	4	5,216,163	1,304,041	206.65	0.000	HS
X₁	1	854,046	854,046	135.34	0.000	HS
X₄	1	4,249,081	4,249,081	673.36	0.000	HS
X₅	1	110,335	110,335	17.49	0.001	HS
X₆	1	2700	2700	0.43	0.525	NS^**
Square	4	2,419,235	604,809	95.85	0.000	HS
X₁*X₁	1	133,961	133,961	21.23	0.001	HS
X₄*X₄	1	2,264,809	2,264,809	358.91	0.000	HS
X₅*X₅	1	117,986	117,986	18.7	0.001	HS
X₆*X₆	1	19,404	19,404	3.08	0.105	NS
2-Way interaction	6	37,188	6198	0.98	0.478	NS
X₁*X₄	1	42	42	0.01	0.936	NS
X₁*X₅	1	16,684	16,684	2.64	0.130	NS
X₁*X₆	1	5280	5280	0.84	0.378	NS
X₄*X₅	1	367	367	0.06	0.813	NS
X₄*X₆	1	2567	2567	0.41	0.536	NS
X₅*X₆	1	12,248	12,248	1.94	0.189	NS
Residual error	12	75,723	6310
Lack-of-fit	10	68,366	6837	1.86	0.400	NS
Pure error	2	7356	3678
Total	26	7,748,308

ISSN: 2731-3654