Identification of key transcription factors, including DAL80 and CRZ1, involved in heat and ethanol tolerance in Saccharomyces cerevisiae

Chen, Rong-Rong; Wang, Li; Ji, Xue-Xue; Xie, Cai-Yun; Tang, Yue-Qin

doi:10.1186/s13068-025-02653-2

Biotechnology for Biofuels and Bioproducts

Table 3 Fermentation performance of engineered strains at 43 °C with 150 g/L glucose

From: Identification of key transcription factors, including DAL80 and CRZ1, involved in heat and ethanol tolerance in Saccharomyces cerevisiae

	Consumed glucose (g/L)	Improvement (%)	Ethanol concentration (g/L)	Improvement (%)	Ethanol yield (g/g consumed glucose)
KF-7	109.44 ± 4.08	–	45.87 ± 2.32	–	0.42 ± 0.03
KF7ΔYAP1	119.68 ± 1.96**	9.36	54.18 ± 0.16***	18.12	0.45 ± 0.01
KF7ΔRPH1	113.50 ± 1.62	3.71	46.25 ± 0.13	0.84	0.41 ± 0.01
KF7ΔCRZ1	120.47 ± 3.46**	10.07	56.17 ± 0.80***	22.46	0.47 ± 0.01*
KF7ΔGIS1	123.93 ± 1.59**	13.24	59.99 ± 0.66****	30.79	0.48 ± 0.01**
KF7ΔPUT3	84.13 ± 0.27****	− 23.13	38.24 ± 2.09**	− 16.63	0.45 ± 0.03
KF7ΔMIG1	93.95 ± 1.74***	− 14.15	40.36 ± 0.42**	− 11.99	0.43 ± 0.01
KF7ΔPHD1	108.11 ± 1.67	− 1.21	42.96 ± 4.56	− 6.33	0.40 ± 0.04
KF7ΔOPI1	99.01 ± 0.08**	− 9.53	43.90 ± 0.86	− 4.29	0.44 ± 0.01
KF7ΔYJL206C	108.07 ± 3.15	− 1.25	46.61 ± 2.85	1.62	0.43 ± 0.03
KF7ΔDAL80	112.95 ± 0.64	3.21	47.38 ± 0.99	3.30	0.42 ± 0.01
KF7ΔUGA3	106.96 ± 6.06	− 2.26	45.50 ± 2.57	− 0.80	0.43 ± 0.01
KF7ΔIME1	100.57 ± 3.31*	− 8.10	48.89 ± 0.30	6.59	0.49 ± 0.02*
KF7ΔTOS8	118.50 ± 1.58*	8.28	49.75 ± 1.89	8.47	0.42 ± 0.02
KF7YAP1	94.51 ± 2.17**	− 13.64	39.66 ± 0.59**	− 13.54	0.42 ± 0.01
KF7RPH1	108.53 ± 0.67	− 0.83	43.29 ± 1.30	− 5.63	0.40 ± 0.01
KF7CRZ1	110.02 ± 2.33	0.53	50.26 ± 1.22*	9.58	0.46 ± 0.01
KF7GIS1	92.76 ± 0.03***	− 15.24	43.53 ± 0.38	− 5.10	0.47 ± 0.01*
KF7PUT3	101.38 ± 1.34*	− 7.37	42.38 ± 0.05	− 7.61	0.42 ± 0.01
KF7MIG1	124.04 ± 2.86**	13.35	54.31 ± 1.29**	18.41	0.44 ± 0.01
KF7PHD1	87.11 ± 3.90***	− 20.41	37.64 ± 0.11***	− 17.95	0.43 ± 0.02
KF7OPI1	121.41 ± 0.58**	10.94	51.24 ± 0.11**	11.72	0.42 ± 0.01
KF7YJL206C	89.87 ± 2.81***	− 17.88	40.69 ± 2.78*	− 11.30	0.45 ± 0.02
KF7DAL80	124.33 ± 1.35***	13.61	53.51 ± 0.40**	16.67	0.43 ± 0.01
KF7UGA3	113.59 ± 2.77	3.79	48.14 ± 1.74	4.96	0.42 ± 0.01
KF7IME1	101.70 ± 0.92*	− 7.08	46.64 ± 0.71	1.69	0.46 ± 0.01
KF7TOS8	118.46 ± 2.88*	8.24	51.44 ± 1.90*	12.15	0.43 ± 0.01
KF7IFH1	111.42 ± 7.19	1.81	47.16 ± 3.31	2.82	0.42 ± 0.01

All data were calculated based on 24 h of fermentation. Values indicate mean ± standard deviation of three biological replications. Statistical significance was determined using an independent samples t test. Difference is indicated as significant (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001) when compared to the control strain KF-7

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