Articles
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Citation: Biotechnology for Biofuels and Bioproducts 2025 18:4
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Batch and semi-continuous fermentation with Parageobacillus thermoglucosidasius DSM 6285 for H2 production
Parageobacillus thermoglucosidasius is a facultatively anaerobic thermophile that is able to produce hydrogen (H2) gas from the oxidation of carbon monoxide through the water–gas shift reaction when grown under a...
Citation: Biotechnology for Biofuels and Bioproducts 2025 18:3 -
Carbohydrate conversion in spent coffee grounds: pretreatment strategies and novel enzymatic cocktail to produce value-added saccharides and prebiotic mannooligosaccharides
Spent coffee grounds (SCG) are the most abundant waste byproducts generated from coffee beverage production worldwide. Typically, these grounds are seen as waste and end up in landfills. However, SCG contain v...
Citation: Biotechnology for Biofuels and Bioproducts 2025 18:2 -
Metabolic engineering of Yarrowia lipolytica for the production and secretion of the saffron ingredient crocetin
Crocetin is a multifunctional apocarotenoid natural product derived from saffron, holding significant promises for protection against various diseases and other nutritional applications. Historically, crocetin...
Citation: Biotechnology for Biofuels and Bioproducts 2025 18:1 -
Enhancing D-lactic acid production by optimizing the expression of D-LDH gene in methylotrophic yeast Komagataella phaffii
Currently, efficient technologies producing useful chemicals from alternative carbon resources, such as methanol, to replace petroleum are in demand. The methanol-utilizing yeast, Komagataella phaffii, is a promi...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:149 -
Strategies and tools to construct stable and efficient artificial coculture systems as biosynthetic platforms for biomass conversion
Inspired by the natural symbiotic relationships between diverse microbial members, researchers recently focused on modifying microbial chassis to create artificial coculture systems using synthetic biology too...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:148 -
Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streams
Biorefineries usually focus on the production of low-value commodities, such as bioethanol, platform chemicals or single cell protein. Shifting production to bioactive compounds, such as antimicrobial peptides...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:147 -
Pyrophosphate-free glycolysis in Clostridium thermocellum increases both thermodynamic driving force and ethanol titers
Clostridium thermocellum is a promising candidate for production of cellulosic biofuels, however, its final product titer is too low for commercial application, and this may be due to thermodynamic limitations in...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:146 -
Biofilm application for anaerobic digestion: a systematic review and an industrial scale case
Biofilm is a syntrophic community of microorganisms enveloped by extracellular polymeric substances and displays remarkable adaptability to dynamic environments. Implementing biofilm in anaerobic digestion has...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:145 -
Reduction of nicotine content in tobacco through microbial degradation: research progress and potential applications
Originally native to South America, tobacco and is now distributed worldwide as a major cash crop. Nicotine is the main harmful component of tobacco leaves, cigarette smoke and tobacco waste, which severely af...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:144 -
Improving productivity of citramalate from CO2 by Synechocystis sp. PCC 6803 through design of experiment
Cyanobacteria have long been suggested as an industrial chassis for the conversion of carbon dioxide to products as part of a circular bioeconomy. The slow growth, carbon fixation rates, and limits of carbon p...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:143 -
Quantitative physiology and biomass composition of Cyberlindnera jadinii in ethanol-grown cultures
Elimination of greenhouse gas emissions in industrial biotechnology requires replacement of carbohydrates by alternative carbon substrates, produced from CO2 and waste streams. Ethanol is already industrially pro...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:142 -
Optimizing hexanoic acid biosynthesis in Saccharomyces cerevisiae for the de novo production of olivetolic acid
Medium chain fatty acids (MCFAs) are valuable platform compounds for the production of biotechnologically relevant chemicals such as biofuels and biochemicals. Two distinct pathways have been implemented in th...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:141 -
Thermobifida fusca Cel6B moves bidirectionally while processively degrading cellulose
Cellulose, an abundant biopolymer, has great potential to be utilized as a renewable fuel feedstock through its enzymatic degradation into soluble sugars followed by sugar fermentation into liquid biofuels. Ho...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:140 -
Valorization of milling byproducts and ergot-sclerotia-contaminated rye via clostridial ABE fermentation
Acetone–butanol–ethanol (ABE) fermentation by solventogenic clostridia can be harnessed to produce CO2 emission neutral bio-based 1-butanol, a valuable compound with a broad range of applications, e.g., in indust...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:139 -
Biochemical and inhibitor analysis of recombinant cellobiohydrolases from Phanerochaete chrysosporium
The demand for greener energy sources necessitates the development of more efficient processes. Lignocellulosic biomass holds significant potential for biofuels production, but improvements in its enzymatic de...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:138 -
Heat of reaction in individual metabolic pathways of yeast determined by mechanistic modeling in an insulated bioreactor
The yeast Saccharomyces cerevisiae, commonly used in industry, exhibits complex metabolism due to the Crabtree effect, fermenting alcohol even under aerobic conditions when glucose exceeds 0.10-0.15 g/L. The heat...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:137 -
Sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stover
White rot fungi produce various reactive oxygen species and ligninolytic enzymes for lignocellulose deconstruction. However, their interactions during the deconstruction of lignocellulosic structural barriers ...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:136 -
Enhancement of non-oleaginous green microalgae Ulothrix for bio-fixing CO2 and producing biofuels by ARTP mutagenesis
Oleaginous green microalgae are often mentioned in algae-based biodiesel industry, but most of them belong to specific genus (Chlorella, Scenedesmus, Botryococcus and Desmodesmus). Thus, the microalgal germplasm ...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:135 -
Potential, economic and ecological benefits of sweet sorghum bio-industry in China
Sweet sorghum (Sorghum bicolor) displays an excellent potential to serve as a non-food bioenergy feedstock for bioethanol production in China due to its high potential yield on marginal lands. However, few studie...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:134 -
Production and characterization of novel/chimeric sophorose–rhamnose biosurfactants by introducing heterologous rhamnosyltransferase genes into Starmerella bombicola
Glycolipid biosurfactant, sophorolipids (SLs) and rhamnolipids (RLs) can be widely used in agriculture, food and chemical industries. The different physicochemical properties of SLs and RLs, such as hydrophili...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:133 -
Simultaneous saccharification and fermentation for d-lactic acid production using a metabolically engineered Escherichia coli adapted to high temperature
Escherichia coli JU15 is a metabolically engineered strain capable to metabolize C5 and C6 sugars with a high yield of d-lactic acid production at its optimal growth temperature (37 °C). The simultaneous sacchari...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:132 -
Expanding the biosynthesis spectrum of hydroxy fatty acids: unleashing the potential of novel bacterial fatty acid hydratases
Hydroxy fatty acids represent an emerging class of compounds with promising applications in the chemical, medicinal and functional food sectors. The challenges associated with their chemical synthesis have spu...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:131 -
Engineering the L-tryptophan metabolism for efficient de novo biosynthesis of tryptophol in Saccharomyces cerevisiae
Tryptophol (IET) is a metabolite derived from L-tryptophan that can be isolated from plants, bacteria, and fungi and has a wide range of biological activities in living systems. Despite the fact that IET biosy...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:130 -
Biofuel production: exploring renewable energy solutions for a greener future
Biofuel production has emerged as a leading contender in the quest for renewable energy solutions, offering a promising path toward a greener future. This comprehensive state-of-the-art review delves into the ...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:129 -
Engineered reduction of S-adenosylmethionine alters lignin in sorghum
Lignin is an aromatic polymer deposited in secondary cell walls of higher plants to provide strength, rigidity, and hydrophobicity to vascular tissues. Due to its interconnections with cell wall polysaccharide...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:128 -
Elucidating Thermothielavioides terrestris secretome changes for improved saccharification of mild steam-pretreated spruce
The efficient use of softwood in biorefineries is hampered by its recalcitrance to enzymatic saccharification. In the present study, the fungus Thermothielavioides terrestris LPH172 was cultivated on three steam-...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:127 -
The overexpression of the switchgrass (Panicum virgatum L.) genes PvTOC1-N or PvLHY-K affects circadian rhythm and hormone metabolism in transgenic Arabidopsis seedlings
Switchgrass (Panicum virgatum L.) is a perennial C4 warm-season grass known for its high-biomass yield and wide environmental adaptability, making it an ideal bioenergy crop. Despite its potential, switchgrass se...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:126 -
Efficient enhancement of the antimicrobial activity of Chlamydomonas reinhardtii extract by transgene expression and molecular modification using ionizing radiation
Ionizing radiation has been used for mutagenesis or material modification. The potential to use microalgae as a platform for antimicrobial production has been reported, but little work has been done to advance...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:125 -
Process scale-up simulation and techno-economic assessment of ethanol fermentation from cheese whey
Production of cheese whey in the EU exceeded 55 million tons in 2022, resulting in lactose-rich effluents that pose significant environmental challenges. To address this issue, the present study investigated c...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:124 -
Microwave-assisted organic acids and green hydrogen production during mixed culture fermentation
The integration of anaerobic digestion into bio-based industries can create synergies that help render anaerobic digestion self-sustaining. Two-stage digesters with separate acidification stages allow for the ...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:123 -
Divergent roles of ADP-ribosylation factor GTPase-activating proteins in lignocellulose utilization of Trichoderma guizhouense NJAU4742
The ability of lignocellulose degradation for filamentous fungi is always attributed to their efficient CAZymes system with broader applications in bioenergy development. ADP-ribosylation factor GTPase-activat...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:122 -
Engineering Escherichia coli for utilization of PET degraded ethylene glycol as sole feedstock
From both economic and environmental perspectives, ethylene glycol, the principal constituent in the degradation of PET, emerges as an optimal feedstock for microbial cell factories. Traditional methods for co...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:121 -
Discovery of alkaline laccases from basidiomycete fungi through machine learning-based approach
Laccases can oxidize a broad spectrum of substrates, offering promising applications in various sectors, such as bioremediation, biomass fractionation in future biorefineries, and synthesis of biochemicals and...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:120 -
Clostridium autoethanogenum alters cofactor synthesis, redox metabolism, and lysine-acetylation in response to elevated H2:CO feedstock ratios for enhancing carbon capture efficiency
Clostridium autoethanogenum is an acetogenic bacterium that autotrophically converts carbon monoxide (CO) and carbon dioxide (CO2) gases into bioproducts and fuels via the Wood–Ljungdahl pathway (WLP). To facilit...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:119 -
The impact of the carbohydrate-binding module on how a lytic polysaccharide monooxygenase modifies cellulose fibers
In recent years, lytic polysaccharide monooxygenases (LPMOs) that oxidatively cleave cellulose have gained increasing attention in cellulose fiber modification. LPMOs are relatively small copper-dependent redo...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:118 -
Furfural tolerance of mutant Saccharomyces cerevisiae selected via ionizing radiation combined with adaptive laboratory evolution
Lignocellulose is a renewable and sustainable resource used to produce second-generation biofuel ethanol to cope with the resource and energy crisis. Furfural is the most toxic inhibitor of Saccharomyces cerevisi...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:117 -
A scalable, chromatography-free, biocatalytic method to produce the xyloglucan heptasaccharide XXXG
Xyloglucan oligosaccharides (XyGOs) are highly branched, complex carbohydrates with a variety of chemical and biotechnological applications. Due to the regular repeating pattern of sidechain substitution of th...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:116 -
Microbial conversion of ethanol to high-value products: progress and challenges
Industrial biotechnology heavily relies on the microbial conversion of carbohydrate substrates derived from sugar- or starch-rich crops. This dependency poses significant challenges in the face of a rising pop...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:115 -
Hydrothermal conditioning of oleaginous yeast cells to enable recovery of lipids as potential drop-in fuel precursors
Lipids produced using oleaginous yeast cells are an emerging feedstock to manufacture commercially valuable oleochemicals ranging from pharmaceuticals to lipid-derived biofuels. Production of biofuels using ol...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:114 -
Bubbling insights: unveiling the true sophorolipid biosynthetic pathway by Starmerella bombicola
The yeast Starmerella bombicola is renowned for its highly efficient sophorolipid production, reaching titers and productivities of (over) 200 g/L and 2 g/(L h), respectively. This inherent efficiency has led to ...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:113 -
Rapid measurement of soluble xylo-oligomers using near-infrared spectroscopy (NIRS) and multivariate statistics: calibration model development and practical approaches to model optimization
Rapid monitoring of biomass conversion processes using techniques such as near-infrared (NIR) spectroscopy can be substantially quicker and less labor-, resource-, and energy-intensive than conventional measur...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:112 -
Establishment of Saccharomyces cerevisiae as a cell factory for efficient de novo production of monogalactosyldiacylglycerol
Monogalactosyldiacylglycerol (MGDG), a predominant photosynthetic membrane lipid derived from plants and microalgae, has important applications in feed additives, medicine, and other fields. The low content an...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:111 -
Efficiency of acetate-based isopropanol synthesis in Escherichia coli W is controlled by ATP demand
Due to increasing ecological concerns, microbial production of biochemicals from sustainable carbon sources like acetate is rapidly gaining importance. However, to successfully establish large-scale production...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:110 -
Exploring the stress response mechanisms to 2-phenylethanol conferred by Pdr1p mutation in Saccharomyces cerevisiae
The 2-phenylethanol (2-PE) tolerance phenotype is crucial to the production of 2-PE, and Pdr1p mutation can significantly increase the tolerance of 2-PE in Saccharomyces cerevisiae. However, its underlying molecu...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:109 -
Strain and model development for auto- and heterotrophic 2,3-butanediol production using Cupriavidus necator H16
The production of platform chemicals from renewable energy sources is a crucial step towards a post-fossil economy. This study reports on the production of acetoin and 2,3-butanediol heterotrophically with fru...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:108 -
Metabolic and tolerance engineering of Komagataella phaffii for 2-phenylethanol production through genome-wide scanning
2-Phenylethanol (2-PE) is one of the most widely used spices. Recently, 2-PE has also been considered a potential aviation fuel booster. However, the lack of scientific understanding of the 2-PE biosynthetic p...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:107 -
Transcriptomic and metabolic changes in Trichoderma reesei caused by mutation in xylanase regulator 1 (xyr1)
Trichoderma reesei is known for its ability to produce large amounts of extracellular proteins and is one of the most important industrially used filamentous fungus. Xylanase regulator 1 (XYR1) is the master regu...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:106 -
An integrated QTL and RNA-seq analysis revealed new petal morphology loci in Brassica napus L.
Rapeseed (Brassica napus L.) is one of the most important oil crops and a wildly cultivated horticultural crop. The petals of B. napus serve to protect the reproductive organs and attract pollinators and tourists...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:105 -
Enzymatic hydrolysis of waste streams originating from wastewater treatment plants
Achieving climate neutrality is a goal that calls for action in all sectors. The requirements for improving waste management and reducing carbon emissions from the energy sector present an opportunity for wast...
Citation: Biotechnology for Biofuels and Bioproducts 2024 17:104